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KMID : 0545120080180111858
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 11 p.1858 ~ p.1861
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Rapid and Sensitive Detection of Listeria monocytogenes Using a PCR-Enzymelinked Immunosorbent Assay
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Kim Hye-Jin
Cho Jae-Chang
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Abstract
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A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for the rapid and sensitive detection of L. monocytogenes. PCR primers generating a 132-bp amplicon and a capture probe able to hybridize to the PCR amplicon were designed based on the L. monocytogenes-specific hly gene encoding listeriolysin. The detection limit of PCR-ELISA for L. monocytogenes was determined to be as low as 10 cells per PCR reaction, and this level of detection was achieved within 5 h. These results indicate that the PCR-ELISA provides a valuable tool for the rapid and sensitive detection of L. monocytogenes for the ready-to-eat food industry.
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KEYWORD
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PCR-ELISA, Listeria monocytogenes
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